According to the structure characteristics of multiplayer binding high pressure vessels, acoustic emission linear location method is used to detect deep active defects in hoop weldments. At the same time, triangle location and zone location methods are used to inspect the whole cylinder. The result shows that multiplayer binding vessel with excellent integrity presents elastic characteristic like monolayer vessel in the second boosting period. In the second holding period, there are only few AE hits and no obvious acoustic sources. To the vessel with active defects, latent dangerous sources can be located, which can not be located by other testing methods. All of these approve that acoustic emission technology can not only be used on the testing and assessment of monolayer pressure vessel, but also on multiplayer binding high pressure vessel.